Course title: Basic flow cytometry and cell sorting
Aim and content: The aim of the course is to introduce students to flow cytometry. The course will take you through basic knowledge of how to design and choose the right experiment, how to operate the instrument, and some of the pitfalls in flow cytometry. Several techniques will be demonstrated and the student will get a chance to work with these on our different instruments (FACSCalibur, LSR-II, and Aria-II).
The course is focused on the use of Diva software, and to some extent CellQuest because of our choice of instruments, but independent software such as FlowLogic will also be demonstrated.
During the course some exercises will require preparation from day to day, since presentation / discussion with the group is needed.
After the course each student will be granted an hour's assistant, as help to design or troubleshoot their own experiment, if needed. This hour can be arranged later, if needed.
The course is part of the program from Immunology and Infectious Diseases PhD school.
Participants: PhD students and people on a similar level, no previous skills in flow cytometry are needed.
Form: E-learning lectures, practical and theoretical exercises. The course has a follow up where each student can get help designing there own experiments.
ECTS-credits: 5.1 point (Spring 2016)
Course director: Jan Pravsgaard Christensen; ISIM; 35327873; firstname.lastname@example.org
Teachers: Jan Pravsgaard Christensen as well as students/technicians at the institute
Dates: 2 weeks of e-learning before the practical course that can be done at home followed by 2½ days practical course in house. Se individual posting on the PhD schools homepage.
Place: The Panum Institute, Blegdamsvej 3c, DK-2200 Copenhagen N.
Jan P. Christensen
Institute of Immunology and Microbiology
Blegdamsvej 3c, Maersk Tower 07-13-59
Registration: See individual posting on the PhD school's homepage.
No admission after deadline. Admission for Ph.D. students will be allocated on a first-come, first-served basis. Applications from external participants will be considered after the closing date. The application must be sent via the web-application.